CoolLED pE-300 Ultra

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The CoolLED pE-300ultra is a highly controllable fluorescence microscopy Illumination System which offers intense, broad-spectrum LED illumination for imaging most common fluorophores.  Fast events can be captured with microsecond switching via multiple TTL inputs, and the ability to mount inline excitation filters allows fast, flexible filter configurations.  This, when paired with today’s high-performance multi-band filter sets, facilitates imaging traditionally done via a white Light Source and a filter wheel, with all the benefits of LEDs.

LED technology is quickly evolving, and since 2017 our new generation has double the irradiance at the sample plane, and for even higher irradiance is available as a Direct Fit model (which also reduces the cost to buy and run thanks to fewer consumables). With ACT Label certification, it’s even an environmentally friendly option for illumination.


Light Source, Control Pod, set of three Excitation Filter Holders (25mm dia.), Power Supply, Plug (Direct Fit version includes microscope adapter).


Please confirm microscope make & model at Checkout
Please confirm microscope make & model at Checkout

The pE-300ultra is the most controllable member of the pE-300 Series.  In addition to the many features of the pE-300lite and the pE-300white, the system offers precise control over wavelength irradiance and shuttering.  Until now these benefits have only been accessible to users of high end, highly flexible LED microscope illuminators such as the CoolLED pE-4000.

It allows adjustment of output in 1% steps, giving precise control.  The broad spectrum covers everyday fluorophores: DAPI, CFP, Aqua, FITC, TRITC, TxRed, Cy5 and many more.

It is now possible to view and image your samples brighter than ever

As part of the award-winning pE-300 Series, the enhanced pE-300ultra now delivers double the irradiance at the microscope sample plane.

With the new enhanced pE-300ultra, all three channels at full power use just 60 Watts. That is almost a quadruple efficiency increase. This compares to other LED technology that uses 120 to 350 Watts. This leap forward in technology makes LEDs more attractive when compared to old Mercury or Metal Halide technology.  Where “green” funding is available, the reduction in institutional energy usage improves the return on investment.

Capturing high-speed events

The pE-300ultra offers triggering from multiple TTL inputs which, coupled with the ability to mount inline excitation filters, provides microsecond switching of pre-filtered excitation light.  This, when paired with today’s high performance multi-band filter sets, facilitates imaging traditionally done using a white Light Source and a filter wheel, with all the benefits of LED – and most excitingly at speeds not previously so affordable. For live cell imaging applications, events can now be captured that might previously have been missed.


Fast multi-channel imaging without pixel shift

Fluorophores can be viewed either individually or in combination, without filter cube changes.  This makes the pE-300ultra ideal for use with multi-band filter sets as the screening process can be simplified when fewer filter cubes are used.  Independent control of the three LED channels means that the user can control the level of excitation of each fluorophore independently on a multi-stained sample, potentially removing the need for single-band filter sets altogether.

Sequence runner for multi-channel excitation

It is now possible to run through a sequence of excitation channels using a camera which has only a single TTL-out, thanks to the Sequence Runner mode. The order of each fluorophore to be captured is defined via the simple Control Pod, with the pE-300ultra Light Source accepting a single TTL output from the experiment set-up’s camera to initiate the step-through of a sequence of excitation channels.  This feature is independent of the individual channel TTL inputs on the Light Source.

Direct fit or liquid light guide

There are two pE-300ultra configuration options: direct fit or liquid light guide. The Direct Fit variant delivers higher irradiance and reduces the cost to buy and run thanks to fewer consumables. As a replacement to a metal halide source on a confocal microscope, the Liquid Light Guide model is particularly suitable for pre-aligning samples before scanning commences.  In this way, photo bleaching of the sample can be minimised when LED illumination is used. More information on the available variants can be found under specifications .

Award winning technology

CoolLED’s pE-300 Series was the 2017 winner of the “Go Beyond Award” for “Excellence in sustainability in laboratory and other high technology facilities”. The International Institute for Sustainable Laboratories (I2SL) runs a unique awards program honouring organisations, individuals, products, and projects that are advancing sustainable, high-performance facilities.

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CoolLED pE-300 Series Brochure

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